Our result showed that ICC cells in the Gal-3-shRNA group exhibited 9.81 and 24.21% decreased migration ability compared with the control group after 24 and 48 h, respectively, which was well correlated with the result of Transwell assay showing that ICC cells transfected with Gal-3-shRNA exhibited decreased migration ability of crossing the membrane in contrast to the control group. The gene discussed is LGALS3; the disease is intrahepatic cholangiocarcinoma.