To further explore whether EBLN3P has a functional effect on tumor metastasis ability, we performed a scratch-wound healing assay and observed that compared with SW480 and HCT116 cells transfected with sh-NC, those transfected with sh-1 or sh-2 showed a significantly increased migration 24 h after transfection (Figure 4A). This evidence concerns the gene EBLN3P and neoplasm.