The RT-PCR and western blotting results showed that the mRNA and protein expression of BRD7 were both markedly upregulated in CRC cell lines when compared with NCM460 (Figure 2A), and HCT116 and SW620 cells were therefore chosen as the cell models for investigation of BRD7 function and mechanism based on their moderate expression among these CRC cell lines. The gene discussed is BRD7; the disease is colorectal carcinoma.