Further, orally administered Salmonella ΔmsbB in combination with PI+TGFβ+IL10 and anti-CD3 mAb prevented the hyperglycemia and normalized blood glucose levels in 80% of mice for more than 100 days post-vaccination (Figure 5C) compared to 20% of mice, given vehicle alone, and 60% of the mice treated with either PI+TGFβ or PI+IL10 and anti-CD3 mAb (Figure 5C). This evidence concerns the gene TGFB1 and Hyperglycemia.