To investigate whether tanshinone compounds inhibit tumor cell growth via FHIT-dependent apoptosis pathway, we created FHIT knockout (KO) colorectal HCT116 cells by CRISPR/Cas9 mediated genome editing, genomic DNA sequence revealed that exon5 to exon6 of the FHIT gene was deleted (Fig. S2A), and the depletion of FHIT protein was confirmed by western blotting (Fig. S2B). Here, FHIT is linked to neoplasm.