Two p53 wild-type leukemia lines (Molm-13, Molt-4), were treated with three topoisomerase II inhibitors (daunorubicin, idarubicin and etoposide) for 4 h in the presence or absence of M3814 and mRNA levels of p21 and Puma were determined by qPCR (Fig. 2B). The gene discussed is TP53; the disease is leukemia.