To validate the above results and explore whether WSX1 is associated with HCC development, we further analyzed WSX1 expression in human liver tissue microarrays (BC03116a and HLiv-HCC180Sur-03), which included 130 cases of HCC, 17 normal tissue samples from healthy donors, and 103 normal tumor-adjacent liver tissues (NAT). The gene discussed is IL27RA; the disease is hepatocellular carcinoma.