In summary, the present study demonstrated that (1) inhibition of glycolysis suppressed the expression of proinflammatory genes in LPS-activated microglial cells in vitro and in vivo; (2) inhibition of NAD+/SIRT1/p65 acetylation was involved in the anti-neuroinflammatory activity of the glycolytic inhibitors (Fig. 9); (3) AMPK/mTOR/IKK signaling was involved in the anti-neuroinflammatory response of the glycolytic inhibitors (Fig. 9); and (4) glycolytic inhibitor 2-DG exhibited neuroprotective effects in LPS- and MPTP- mouse PD models. Here, RELA is linked to Parkinson disease.