Furthermore, analysis of canonical signaling pathways pointed to the important role of G2/M DNA damage checkpoint regulation, iron homeostasis signaling pathway, tight junction signaling, sirtuin signaling pathway, α-adrenergic and β-adrenergic signaling, HIPPO signaling, mTOR signaling, amyotrophic lateral sclerosis signaling, axonal guidance signaling, and growth hormone signaling, among others (Fig. 3C, Additional file 1: Table S11). Here, GH1 is linked to amyotrophic lateral sclerosis.