Besides, there are two additional genes related to muscle development at different stages of embryos, including VASH2 and TUBAL3. Among them, VASH2 was belonged to the Vasohibin family, which is involved in angiogenesis [39, 40] and verified as a biomarker for the esophageal squamous cell carcinoma [41]. The gene discussed is TUBAL3; the disease is esophageal squamous cell carcinoma.