To further reveal the underlying mechanism responsible for the coordinate upregulation of DPP10-AS1 and DPP10 in lung cancer, we used the DNA methyltransferase inhibitor 5-azacytidine to determine the effect of methylation on the expression of DPP10-AS1 and DPP10. The relative expression of DPP10-AS1 was markedly upregulated with increasing 5-azacytidine concentration in both SPC-A1 and NCI-H1299 cells (Figure 8D). The gene discussed is DPP10; the disease is lung cancer.