As shown in Fig. 4A, B, in both glioma cell lines, RNF139 overexpression dramatically decreased the ratio of p-PI3K/PI3K and p-AKT/AKT; on the contrary, RNF139 knockdown induced by either shRNA1-RNF139 or shRNA2-RNF139 elevated the ratio of p-PI3K/PI3K and p-AKT/AKT. Here, PIK3CA is linked to central nervous system cancer.