Interestingly, in line with the expression pattern of mono‐positive maker, higher ratio of multi‐positive (CD44/PROM1, CD44/EPCAM and CD44/PROM1/EPCAM) tumor cells was found in organoid HCC272 in comparison with other three HCC organoids (Figure 3B), suggesting that the abnormal enrichment of CD44+ cells might be another responsible for its drug resistance. This evidence concerns the gene EPCAM and hepatocellular carcinoma.