PPARGC1A and ischemia: As shown in Figure 3(a), after 75 min of ischemia and 6 h of reperfusion, the activation of the PPARs in the Ad-GFP-transduced I/R group was mildly decreased, and PGC-1α overexpression or knockdown attenuated or aggravated this reduction, respectively, in comparison with Ad-GFP-transduced sham mice (Figure 3(a)).