ICI monotherapy did not stimulate CD3+ infiltration (Fig. 2d) or suppress the population of myeloid cells in the tumors (Fig. 2e); it did not enhance the radio of CD8+/CD3+ T cells or affect Ki67, interferon-γ (IFN-γ), and CD69 expression in CD3+ T cells (Fig. 2f, g), suggesting that ICIs fail to induce activation of T cells in GBM. This evidence concerns the gene CD69 and glioblastoma.