GBM was induced in Ntv-a;Ink4a-Arf−/−;Ptenfl/fl;LSL-Luc donor mice by RCAS (replication-competent avian sarcoma-leukosis virus long terminal repeat with splice acceptor)-mediated gene transfer, followed by orthotopic implantation of tumor cells into Cdh5-CreERT2;Il6fl/fl mice, in which IL-6 expression is controlled by EC-specific promoter Cdh5. Here, CDKN2A is linked to glioblastoma.