Importantly, a single low-dose (1 μg) of VEGFC mRNA-LNPs effectively reduced paw thickness, clinical score, the fibroadipose area, and Collagen I content compared to the Poly(C) RNA-LNP control 30 and 75 days after disease induction in the experimental secondary lymphedema mouse model after injecting into the contralateral paw of the same animal (Fig. 9a–c, f). The gene discussed is VEGFC; the disease is lymphedema.