We observed a high intra- and inter-tumoral heterogeneity of protein expression, regarding staining intensity and tumor proportion score (TPS) of the immune checkpoint ligands Nectin-2, PVR and PD-L1, as well as receptors PD-1 and TIGIT on tumor cells varied within one tumor segment, as well as between different tumor segments and growth pattern areas (Fig. 2 and 3). This evidence concerns the gene TIGIT and neoplasm.