To test if B16F10‐associated EphB4 activates tumor vascular EphrinB2, we generated syngeneic B16F10 tumors depleted of EphB4 and control tumors by infecting B16F10 cells with either a validated shRNA that effectively depletes EphB4 (Salvucci et al,2015) or with the control vector (pLKO) (Fig 1E and F). This evidence concerns the gene EPHB4 and neoplasm.