To confirm whether the reduced Tgf-β1 was caused by the deletion of RhoA in AT2 cells of Sftpc-cre;RhoAfl/fl mice, we examined Tgf-β1 expression specifically in AT2 cells of the asthma mouse model by coimmunofluorescence staining with both Tgf-β1 and Sftpc. This evidence concerns the gene RHOA and asthma.