Comparing FLT3‐ITD‐positive (n = 98) and FLT3‐ITD‐negative specimens (n = 282) irrespective of sex, we found that GLI2, CCL1, JPH1, MDGA1, RASGRF1, AE000661.37, HOXB‐AS3, IRX5 and RP11‐713C5.1 were all significantly upregulated in FLT3‐ITD‐positive AML, while GPR126 was significantly downregulated. Here, RASGRF1 is linked to acute myeloid leukemia.