COL8A2 and Fuchs endothelial corneal dystrophy: In this study, we demonstrated that intraocular injection of a single adenoviral vector achieved efficient and restricted delivery of CRISPR/Cas9 to adult post-mitotic corneal endothelium, leading to in vivo knockdown of mutant Col8a2 with long-term preservation of corneal endothelial density, structure, and function in the early-onset Fuchs’ dystrophy mouse model.