TIL-iPS-T were co-cultured with GFP-transduced cancer spheroids at a ratio of 1:1 in phenol red-free D-MEM supplemented with 10% fetal bovine serum, 2 mM l-glutamine, 100 U/ml penicillin, 100 ng/ml streptomycin, 100 U/ml IL-2, 5 ng/ml IL-7, and 5 ng/ml IL-15. The gene discussed is IL2; the disease is cancer.