MBD4 and adenocarcinoma: Based on the bulk RNA-seq data from GEM tumors, we found that DNA methyltransferases (Dnmt1, Dnmt3b), demethylase (Tet1), and methyl-CpG binding domain genes (Mbd3 and Mbd4) were differentially upregulated, while Mbd2 and Zbtb4 were downregulated in poorly differentiated foci compared to adenocarcinoma foci (Fig. 7a).