Our data revealed that SRSF1 regulated the apoptosis signaling pathway by participating in the alternative splicing of Mcl-1 and triggered the elevation of Mcl-1L subtype in ESCC, providing the novel insight into the role of SRSF1 in Mcl-1 alternative splicing of ESCC cells. This evidence concerns the gene SRSF1 and esophageal squamous cell carcinoma.