Furthermore, we examined the expression of Mcl-1 at protein level regulated by SRSF1, which showed overexpression or knockdown SRSF1 could further elevate or reduce the levels of Mcl-1 protein in ESCC cells (Fig. 5E and Supplementary Fig. 5A). The gene discussed is SRSF1; the disease is esophageal squamous cell carcinoma.