We confirmed that SRSF1 protein had significant enrichment of DGCR5 in immunoprecipitated TE1 cells lysate with respect to negative control by RNA-binding protein immunoprecipitation (RIP) assays (Fig. 3B), suggesting there was a direct interaction between DGCR5 and SRSF1 protein in ESCC cells. Here, SRSF1 is linked to esophageal squamous cell carcinoma.