Moreover, the ALS-associated mutations R47H-TBK1 and R228H-TBK1 have weaker autophosphorylation activity than WT-TBK1 based on biochemical studies (8), yet they also translocated to damaged mitochondria with the same incidence as WT-TBK1 (Fig. 3 A and B). This evidence concerns the gene TBK1 and amyotrophic lateral sclerosis.