To validate RNA-seq results, qRT-PCR was performed on four myelin genes that were found to be significantly downregulated in DEA: MoBP, Plp1, Mog, and Cnp. Using total RNA from control and IUGR/hyperoxia P14 hemispheres, we found all genes to be significantly downregulated, *p < 0.05 (Fig. 6). This evidence concerns the gene MOBP and fetal growth restriction.