Co-electroporation of VGAT::gal4 or CMV::gal4 with UAS::TVA, and UAS::tRFP into the tectum, followed by injection of SADΔG-EGFP(EnvA) virus 4 d later resulted in infection in the majority of tadpoles (n = 25 tadpoles, 60% infected; Fig. 1F). This evidence concerns the gene LGALS4 and infection.