Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the top seven enriched pathways were the PI3K/AKT signaling pathway, focal adhesion, amoebiasis, extracellular matrix-receptor (ECM-receptor) interaction, protein digestion and absorption, vitamin digestion and absorption, etc. (Fig. 5E). The gene discussed is AKT1; the disease is amebiasis.