The differential bile acids between ICP1 group and cholelithiasis group were TUDCA, UDCA, TCA, CDCA, GUDCA, DCA, CA, and GCDCA, and the concentration of TUDCA, UDCA, TCA, GUDCA, and CA in ICP1 group were higher than cholelithiasis group (P < 0.05), while the concentration of DCA and GCDCA were lower than cholelithiasis group (P < 0.05). Here, ATP8B1 is linked to cholelithiasis.