In this study, we found that the exogenous activation of Wnt signalling using antibody (α‐SOST antibody) or chemicals (LiCl, VPA or CHIR9902) increases β‐catenin (β‐cat) in ameloblastoma, leading to an increase in β‐cathigh and Sox2‐ cells and decrease in β‐catlow and Sox2+ cells, the putative cancer stem cells (CSCs). Here, SOX2 is linked to ameloblastoma.