We also found abnormal TRPC6 activation and high glucose-induced podocyte apoptosis in the DM and HG groups, characterized by increased expression levels of cleaved-caspase-3 and bax/bcl-2 ratios, while FK506 reduced the extent of podocyte damage related to DN and attenuated the elevated expression levels of TRPC6 with improvement in the nephrin expression. Here, CASP3 is linked to liver dysplastic nodule.