Basing on our data, mice continuously fed with HFHC for 16 weeks developed NASH, which replicates many pathophysiological features of human NASH; infiltration of F4/80+ macrophages is observed, as well as the activation of KCs with increased expression of proinflammatory cytokines including IL-6, TNF-α, IL-1β, IL-1α, MCP-1, and GM-CSF to further enhance the inflammatory reaction. The gene discussed is CCL2; the disease is metabolic dysfunction-associated steatohepatitis.