Patel et al. (2017) transduced NY-ESO-1+ Mel624 melanoma cells with a genome-scale CRISPR library of 123,411 sgRNAs, and constructed NY-ESO-1 antigen specific TCR-T cells. Then the transduced melanoma cells were co-incubated with NY-ESO-1 antigen specific TCR-T cells. They found antigen presentation and IFN-γ pathway related genes, such as HLA-A, B2M, TAP1, TAP2, and TAPBP were among the most critical genes in the screen. Besides, the functional loss of APLNR reduced the efficacy of immunotherapy by interacting with JAK1 and modulating IFN-γ responses in tumors (Patel et al., 2017). The gene discussed is B2M; the disease is melanoma.