We down-regulated the expression of DCBLD2 in colorectal cancer cells, and the mRNA and protein levels of transcription factors upstream of EMT signal, ZEB1, ZEB2 and SNAIL significantly decreased, which suppress epithelial genes and activate a mesenchymal expression program (Lin et al., 2013; Kotiyal and Bhattacharya, 2016). The gene discussed is DCBLD2; the disease is colorectal cancer.