SPN and hereditary elliptocytosis: The frequency of HE precursors (CD45−CD43−CD34 + CD31+), hematopoietic cells (both CD43 + CD45− and CD43 + CD45 +), and hematopoietic progenitors (CD45 + CD34 +) as well as the output of clonogenic progenitors detected by hematopoietic CFU assays was analyzed at indicated time points throughout EB development (Figures 1B–E).