The frequency of HE precursors (CD45−CD43−CD34 + CD31+), hematopoietic cells (both CD43 + CD45− and CD43 + CD45 +), and hematopoietic progenitors (CD45 + CD34 +) as well as the output of clonogenic progenitors detected by hematopoietic CFU assays was analyzed at indicated time points throughout EB development (Figures 1B–E). This evidence concerns the gene PECAM1 and hereditary elliptocytosis.