Overall, although a slight trend in favor of protocol 1 was consistently observed, no statistically significant differences were observed in the frequency of either HE precursors; CD43 + CD45−, CD45+, or CD45 + CD34 + hematopoietic derivatives (Figure 1D); or the output of clonogenic progenitors (Figure 1E) at any time point analyzed (from day 7 to day 22 of EB development). Here, CD34 is linked to hereditary elliptocytosis.