SIRT1 and metabolic dysfunction-associated steatohepatitis: We found that (Younossi et al., 2016) hepatic lipid metabolism improved dynamically by activating the SIRT1/AMPK pathway via PREP disruption under HFD feeding conditions; (Saltzman et al., 2018) PREP disruption markedly improved hepatic proinflammatory status progression by inhibiting phosphorylated ERK and p65 as NASH progresses to more severe stages; (Aron-Wisnewsky et al., 2020) PREP disruption improves intestinal dysbiosis and protects against intestinal epithelial barrier damage induced by a HFD.