RUNX1T1 and acute myeloid leukemia: Conversely, although the PCR technique is highly sensitive, its application is limited to a fraction of AML patients who harbored specific genetic aberrations suitable for MRD detection, including RUNX1–RUNX1T1, CBFB–MYH11, and PML–RARα fusions, and NPM1 mutations (5, 11–16).