Conversely, although the PCR technique is highly sensitive, its application is limited to a fraction of AML patients who harbored specific genetic aberrations suitable for MRD detection, including RUNX1–RUNX1T1, CBFB–MYH11, and PML–RARα fusions, and NPM1 mutations (5, 11–16). This evidence concerns the gene MYH11 and acute myeloid leukemia.