For determination of whether miR-486-5p exerted its function in NSCLC by targeting RSK and p70S6K, we constructed RSK and p70S6K overexpression vectors pcDNA3.1-RSK/pcDNA3.1-p70S6K to find out if restoration of RSK and p70S6K expression could rescue the effects of miR-486-5p. This evidence concerns the gene RPS6KB1 and non-small cell lung carcinoma.