We used qRT-PCR to detect the expression of PPFIA4 in colon cancer cell lines (Figure 2A), and found that the level of PPFIA4 was higher in colon cancer cells, including SW403, HT29, CAco-2, DLD1, SW480, HCT116, and COLO320, than that in normal colon cell lines, including NCM460 and CCD-18Co. This evidence concerns the gene PPFIA4 and colonic neoplasm.