We first analyzed the correlation between PPFIA4 and two glycolysis-related genes, ENO2 (enolase 2) and PFKFB3 (6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3), in the TCGA colon cancer database. Here, PFKFB3 is linked to malignant colon neoplasm.