The number of colonies significantly decreased following the administration of chemotherapy drugs (gemcitabine, paclitaxel) (47). Knockdown of TUBB3 expression in PC cells leads to anchorage-independent and -dependent cell growth related to enhanced anoikis (anchorage-independent apoptosis), thus strengthening the link between suppressed TUBB3 and initiation of apoptosis in PC cells. The gene discussed is TUBB3; the disease is pachyonychia congenita.