AKT1 and colorectal cancer: To determine how EGFR and Akt affect the induction of anoikis in HCT116 colorectal cancer cells by SAE, we treated the cells with 20 μM EGFR inhibitor and 20 μM Akt inhibitor (gefitinib and LY294002) with or without SAE (100 μg/mL) for 24 h.