To confirm that apoptosis was induced, we treated HCT116 colorectal cancer cells with 75 and 125 μg/mL SAE for 24 h, and phosphatidylserine (PS) expressed during apoptosis was stained with Annexin V. The staining ratio of PS was identified at each concentration of SAE using a flow cytometer to determine the ratio of apoptosis. This evidence concerns the gene ANXA5 and colorectal cancer.