In addition, from our RNA-seq analyses on U87MG cells cultured in vitro, we found that almost all cancer stem cell marker genes such as Nestin, ID1, FUT4 and L1CAM showed either a significantly decreased or trends of decreased expression (Nanog, CD133 (Prom1), Sox2 and BMI1) after enzalutamide treatment (80 μM) for 48 h. Here, BMI1 is linked to cancer.