In addition, Hmgb2-I also inhibited the number of M1 microglia by co-stained Iba1 and CD16/32, an M1 microglia marker (Fig. S2), and the pro-inflammatory response in stroke mice, as determined by a complete inhibition of the production of IL-1, Ctss, TNF-α, Cxcl-16 and IL-6 (Fig. 3E), but not the production of anti-inflammatory signals such as IL-10 in stroke mice (Fig. 3F). The gene discussed is TNF; the disease is Stroke.