HMGB2 and Stroke: To confirm the relationship between Hmgb2 and Ctss, we first verified the relative expression (R.E) of Ctss in cytosolic fraction (C) = 0.2 ± 0.03 in sham versus 1.26 ± 0.15 in 7 days after stroke (mean ± SEM, F(2,12) = 36.51, ***p < 0.0001, BF ANOVA, Fig. 5B, C) and secreted into extracellular space after stroke onset; the relative expression (R.E) in extracellular space (E) = 0.23 ± 0.04 in sham versus 3.3 ± 0.62 in 7 days after stroke (mean ± SEM, F(2, 12) = 24.54, ***p = 0.0002, BF ANOVA, Fig. 5B, C).