The pCI-L5 vaccine was able to induce partial protection against infection with Leishmania tropica, and the ratio of IFN-γ/IL-4 in the draining lymph nodes (DLNs) of mice of the study group was 2.83 and 3.62 at the second and fourth weeks after the challenge, respectively, and then it stabilized at the value of 5.07 at the sixth week (greater than 1). Here, IL4 is linked to infection.