Therefore, due to the important role of MAP2K1 and P53 proteins in preventing apoptosis, we decided to investigate the effect of endogenous protein expression of MPL with an intestinal cell specific promoter on expression of MAP2K1 and P53 in MPL protein treated human colorectal and breast cancer cell lines, and human normal white blood cells as a control. The gene discussed is MPL; the disease is breast carcinoma.