WEE1 and hydrops fetalis: However, the phosphorylation level of ERK in the mitogen-activated protein kinase signaling pathway was significantly down-regulated by Berberine/ Sitagliptin compared with the HF diet group in the liver tissue (P<0.001) Also, phosphorylation level ERK by Berberine and Sitagliptin alone was significantly down-regulated compared with the HF diet group in the liver tissue (P<0.05, P<0.01, respectively) (Figure 6B).