The immunofluorescence images showed that ZVI@CMC treatment increased the infiltration of anti-tumor M1 macrophages (CD86+) and cytotoxic T cells (CD8+) in the center of tumor lesions (region 2,Figure 6F, H) as compared to the control group which showed predominantly peri-tumor localization of M1 macrophages and CD8+ T cells (region 2,Figure 6E, G). The gene discussed is CD8A; the disease is neoplasm.