In order to systematically investigate the alterations of site-specific glycosylation in the EMT process of HCC, two widely used HCC cell lines SMMC-7721 and HepG2 were treated by HGF to induce their EMT 26-28, and their whole cellular proteins were harvested at six time points after HGF-treatment (0, 6, 12, 24, 48, and 72 h). Here, HGF is linked to hepatocellular carcinoma.