Since glycolic metabolism of HCC cells is controlled by several key rate-limiting enzymes, including hexokinase 2 (HK2), PFKFB3, PFKFB4, phosphofructokinase, liver type (PFKL), and pyruvate kinase M2 (PKM2) 19. This evidence concerns the gene PFKFB3 and hepatocellular carcinoma.