Among commonly mutated genes, LRP1B, TP53, TTN, MUC16, AHNAK2, OBSCN, FLG, PCLO, HMCN1, USH2A, CSMD3, XIRP2, and RYR2 mutations significantly associated with higher TMB in HCC patients (Fig. 2). The gene discussed is AHNAK2; the disease is hepatocellular carcinoma.